ELECTRIC CIGARETTES’S EFFECT TO THE MDA LEVELS IN BLOOD OF WISTAR RAT

Etha Rambung

Abstract


The use of electronic cigarettes (e-cigarettes) has spread widely and increased dramatically among young people aroud the world. Variations in product design, taste, and marketing patterns increase the young people's interest in electronic cigarettes. Even many electric cigarettes are sold in shoppinng cebters and online, so they are easy to reach by teenagers. This study aims to analyze oxidative stress due to exposure to e-cigarettes by assessing the difference in blood MDA levels in the control and treatment groups. The method used is the Posttest Only Control Group Design. Thirty two male wistar rats divided into four treatments goups: K1 (SC (-), EE (-)), K2 (SC (-), EE(-)), P1 (SC(-),  EE (+) 15 times), and P2 (SC (-), EE (+) 30 times). The treatment is given for 5 minutes/ day for 50 days. Termination was carried out on the 50th day using ketamine. Intracardial blood sampling for examination of MDA levels by the TBARS method. The data obtained were tested by Kruskal Wallis with a significance of p <0.05. MDA blood levels in P2 were significantly higher than P1 (p =0,035), K2 (p =0,001) and K1 (p =0,001). This study shows that e-cigarettes can cause oxidative stress in experimental animals.


Keywords


E-cigarettes, Oxidative stress, MDA level

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References


Alexander, L. E. et al. (2015) ‘Electronic cigarettes: The new face of nicotine delivery and addiction’, Journal of Thoracic Disease, 7(8), pp. E248–E251. doi: 10.3978/j.issn.2072-1439.2015.07.37.

Artha, L. P. W. and Kusuma, M. A. P. (2017) Karakteristik Remaja Laki-laki Pengguna Rokok Elektronik di Kota Denpasar Tahun 2017, 4th Indonesian Conference on Tobacco or Health 2017. Jakarta. Available at: http://ictoh-tcscindonesia.com/wp-content/uploads/2018/01/Proceeding-Book-4th-ICTOH.pdf.

Ayala, A., Muñoz, M. F. and Argüelles, S. (2014) ‘Lipid peroxidation: Production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal’, Oxidative Medicine and Cellular Longevity, 2014. doi: 10.1155/2014/360438.

Bitzer, Z. T. et al. (2018) ‘Effect of flavoring chemicals on free radical formation in electronic cigarette aerosols’, Free Radical Biology and Medicine, 120, pp. 72–79. doi: 10.1016/j.freeradbiomed.2018.03.020.

Catala, A. et al. (no date) Lipid peroxidation.

Damayanti, A. (2016) ‘Penggunaan Rokok Elektronik di Komunitas Personal Vaporizer Surabaya’, Jurnal Berkala Epidemiologi, 4(2), pp. 250–261. doi: 10.20473/jbe.v4i2.2016.250–261.

Devhy, N. L. P. and Yundari, A. . I. D. H. (2017) ‘Faktor Yang Berpengaruh Terhadap Perilaku Merokok Konvensional dan Elektrik Pada Remaja Di Kota Denpasar’, Bali Medika Jurnal, 4(2), pp. 63–72.

Ediana, D. (2016) ‘Faktor-Faktor Yang Berhubungan Dengan Perilaku Merokok Di Dalam Rumah Di Kelurahan Tarok’, Human Care Journal, 1(1). doi: 10.32883/hcj.v1i1.163.

Engwa, G. A. (2018) ‘Free Radicals and the Role of Plant Phytochemicals as Antioxidants Against Oxidative Stress-Related Diseases’, Phytochemicals - Source of Antioxidants and Role in Disease Prevention, (November). doi: 10.5772/intechopen.76719.

Ferkol, T. W. et al. (2018) ‘Electronic cigarette use in youths: A position statement of the Forum of International Respiratory Societies’, European Respiratory Journal, 51(5). doi: 10.1183/13993003.00278-2018.

Grana, R. A. (2013) ‘Electronic cigarettes: A new nicotine gateway?’, Journal of Adolescent Health. Elsevier Inc., 52(2), pp. 135–136. doi: 10.1016/j.jadohealth.2012.11.007.

Hajek, P. et al. (2014) ‘Electronic cigarettes: Review of use, content, safety, effects on smokers, and potential for harm and benefit’, Addiction., 109(11), pp. 1801–1810. doi: 10.1111/add.12659.

Khajehnasiri, F. et al. (2013) ‘Total antioxidant capacity and malondialdehyde in depressive rotational shift workers’, Journal of Environmental and Public Health, 2013. doi: 10.1155/2013/150693.

National Academies of Sciences, Engineering, and Medicine, - (2018) Public Health Consequences of E-cigarettes., Public Health Consequences of E-cigarettes. Edited by K. Stratton, L. Y. Kwan, and D. L. Eaton. Washington, DC: The National Academies Press. doi: 10.17226/24952.

Putra, I. G. N. E. et al. (2018) Gambaran Pemahaman, Persepsi, dan Penggunaan Rokok Elektronik pada Siswa Sekolah Menengah Atas di Kota Denpasar, Proceeding Book 4th ICTOH.

Qasim, H. et al. (2017) ‘Impact of electronic cigarettes on the cardiovascular system’, Journal of the American Heart Association, 6(9). doi: 10.1161/JAHA.117.006353.

Sahu, A., Varma, M. and Kachhawa, K. (2015) ‘A Prognostic Study of MDA , SOD and Catalase in Breast Cancer Patients’, 4(5), pp. 2013–2015.

Suryadinata, R. et al. (2019) ‘the Effects of Exposure Duration To Electronic Cigarette Smoke on Differences in Superoxide Dismutase and Malondialdehyde in Blood of Wistar Rats’, International Journal of Current Pharmaceutical Research, pp. 13–16. doi: 10.22159/ijcpr.2019v11i3.34084.

U.S. Department of Health and Human Services, - (2016) E-Cigarette Use Among Youth and Young Adults: A Report of the Surgeon General. Available at: https://doi.org/10.17226/24952.

Young, I. and Woodside, J. (2001) ‘Antioxidants in health and disease’, J Clin Pathol, 54(176), pp. 176–186. doi: 10.1136/jcp.54.3.176.




DOI: http://dx.doi.org/10.32883/hcj.v5i1.636

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